Studies on Isolated Microspore Culture of the Allotriploid Hybrids Between Cabbage and Chinese Cabbage

Microspore culture of 8 allotriploid hybrids between diploid Chinese cabbage (AA, 2n=2x=20) and autotetraploid cabbage (CCCC, 2n=4x=36) was performed in order to create the alien addition lines. The results showed that embryo production frequency of the allotriploid hybrids (ACC) was lower than their parents. In liquid medium NLN with 15% sucrose and 0.1 mg·L-1 6-BA, 0.2 mg·L-1 2,4-D, 0.05 mg·L-1 KT, the 8 lines were different, the highest of embryo production was 0.0250 embryos per bud,the average was 0.0100 embryos per bud.The plant regeneration frequency of embryoids was only 33.3%. The chromosome number of plantlets derived from microspore culture varied from 14 to 27. The morphological characters were diverse among the different plants.