Microspore Culture of Qingmaye Chinese Cabbage and Its Selection and Breeding of New Varieties

The affecting factors and cultural condition of the isolated microspore in Qingmaye Chinese cabbage breeding have been studied. The embryo formation is closely related with genotypes of donor plant; High temperature treatment 33 ℃,24 h can accelerate formation of embryo from microspore, the number of embryo per bud increased 4.5 times as much as that of 25 ℃ constant T. The added exogenous hormone and poly amino acid to culture medium can improve the inductivity of embryoid, increasing averagely 59.64% than that of the contrast. The content of agar increased to 12 g/L in culture medium can raise seedling rate of embryos significantly, 40.5% higher than that of 8 g/L. Double haploid (DH) plant of Qingmaye Chinese cabbage was obtained through microspore culture. Isogonics lines with good characters in DH lines were selected to make up hybridized combinations, and a new variety of Qingmaye Chinese cabbage was bred.