Isolated-Microspore Culture and Plantlet Regeneration of Chinese Cabbage

In order to bring new germ plasm resource quickly and shorten the breeding process of inbred line during the cross breeding of Chinese cabbage,the isolated-microspore culture was carried among 10 excellent Chinese cabbage hybrids.The results showed that by isolated-microspore culture,the double haploid(DH) homozygous plants could be obtained in 1~2 years.Among the 10 genotype,six got the embryoid and embryogenesis success rate was 60%.The differences of embryiod quantities between the highest and the lowest microspore from different genotype were great.The average embryoid quantity per bud of Chunqiubawang was 10.92,while the average embryoid quantity per bud of Tianlv 58 was only 0.17.Among the embryo genotype,five got the strains and the strain inductivity was 83%.More than 20 strains were obtained by investigating in the field and hybrid combinations were trialed in spring 2012.