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Plant Regeneration and Identification of the Allotriploid Hybrids between Chinese Cabbage and Cabbage

Li XiaoFeng

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Abstract

Microspores culture and plant regeneration of the hybrids F1 between autotetraploid Chinese cabbage(AAAA,2n=4x=40) varieties 9401,9402,9403,9404,9405,9406 and diploid Cabbage(CC,2n=2x=18) varieties 9501 were studied.The results showed that the frequency of embryogenesis was different and low among different hybrids.The percentage of plants with 18 chromosomes was 46.7% in the regenerative plants.The occurrence of diverse chromosome associations at diakinesis such as trivalent,bivalent and univalent suggested that the pollen-mother-cell's meiotic chromosome behavior of plant regeneration was complicated.The morphology of the regenerative plants was different.

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Microspores culture and plant regeneration of the hybrids F1 between autotetraploid Chinese cabbage(AAAA,2n=4x=40) varieties 9401,9402,9403,9404,9405,9406 and diploid Cabbage(CC,2n=2x=18) varieties 9501 were studied.The results showed that the frequency of embryogenesis was different and low among different hybrids.The percentage of plants with 18 chromosomes was 46.7% in the regenerative plants.The occurrence of diverse chromosome associations at diakinesis such as trivalent,bivalent and univalent suggested that the pollen-mother-cell's meiotic chromosome behavior of plant regeneration was complicated.The morphology of the regenerative plants was different.

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Available abstract

Microspores culture and plant regeneration of the hybrids F1 between autotetraploid Chinese cabbage(AAAA,2n=4x=40) varieties 9401,9402,9403,9404,9405,9406 and diploid Cabbage(CC,2n=2x=18) varieties 9501 were studied.The results showed that the frequency of embryogenesis was different and low among different hybrids.The percentage of plants with 18 chromosomes was 46.7% in the regenerative plants.The occurrence of diverse chromosome associations at diakinesis such as trivalent,bivalent and univalent suggested that the pollen-mother-cell's meiotic chromosome behavior of plant regeneration was complicated.The morphology of the regenerative plants was different.

Key concepts: Biology, Microspore, Hybrid, Pollen, Meiosis, Ploidy, Bivalent (engine), Botany

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