2009•PubMedRequires access

[MTT assay for detecting 5-fluorouracil chemosensitivity of human breast carcinoma cell line].

Fangxuan Han, Hui Lin, Ling Ru

Abstract

OBJECTIVE: To assess the feasibility of MTT colorimetric assay for testing the in vitro chemosensitivity of breast cancer cells to 5 fluorouracil (5-Fu). METHODS: The chemosensitivity of human breast carcinoma cell lines MCF-7 and MDA-MB-435S to 5-Fu at different concentrations was evaluated with MTT assay. RESULTS: 5-FU treatment resulted in dose-dependent growth inhibition of the breast cancer cells with both low and high metastatic capacities. CONCLUSIONS: MTT assay may help select appropriate chemotherapeutic agents and provides evidence for individualized chemotherapy for breast cancer.

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Key concepts: MTT assay, Chemosensitivity assay, Fluorouracil, Breast carcinoma, Breast cancer, Cell culture, Cancer research, Oncology