Protective Effect of Resveratrol Pretreatment on Cerebral Ischemic Reperfusion Damage in Rats

Objective: To study on the neuroprotection of resveratrol (Res) pretreatment against injury of cerebral ischemic reperfusion (I/R) in rats and study the molecular mechanism of Res against I/R injury. Methods: The rats are divided into three groups randomly, i.e. sham group, I/R (DMSO) group and Res pretreatment group. The global brain ischemia model was constructed with 4-VO occlusion method. After ischemia of 10min /reperfusion of 22h, test the SOD activity and the content of NO, MDA in the hippocampus tissue with kits. The expression of GRP78 was investigated with RT-PCR. Results: The SOD activity in ischemia group was obviously lower than sham group, the content of NO, MDA was higher than sham group; The SOD activity and the content of NO, MDA induced by ischemia could be reversed significantly after Res preconditioning. Ischemia evidently up-regulating the expression of GRP78, the Res pretreatment can effectually inhibit the expression of GRP78 induced by ischemia. Conclusion: Resveratrol can cut off the way of free radicals' production and accumulation through a mechanism which can up-regulate the SOD activity and inhibit the content of NO and MDA. Accordingly this releases the endoplasmic reticulum stress and lowers the expression of GRP78, weakening cerebral ischemia damage.