Effects of Remifentanil Pretreatment on the Brain Tissue MDA Concentration and SOD Activity in Rats induced by Cerebral Ischemia-reperfusion

Objective To observe the effects of remifentanil pretreatment on the brain tissue malondialdehyde(MDA)concentration and superoxide dismutase(SOD)activity in rats induced by cerebral ischemia-reperfusion(CIR) so as to investigate cerebral protection mechanism of remifentanil. Methods A total of 54 adult male Wistar rats were randomly divided into three groups(n=18 each): sham operation group(Sham), ischemia-reperfusion group (IR), and remifentanil pretreatment group(Rem). Four-vessel occlusion was used to establish the global cerebral ischemia reperfusion model in the study groups .An acute experimental cerebral ischemia rats model induced for 15 minutes, all groups were further divided into 3 subgroups according to the reperfusion time (6,12 and 24 h) to test the brain tissue MDA concentration and SOD activity, to observe the morphological changes of rat cerebral by optical microscope. Results Compared with the Sham group, the MDA concentration in the rat brain tissue signifi cantly increased in IR group(P0.05) , SOD activity signifi cantly decreased (P0.05); Compared with the IR group, the MDA concentration in the rat brain tissue in Rem group remarkably decreased (all P0.05), while the SOD activity obviously increased (all P0.05). Conclusion Remifentanil pretreatment shows an neuroprotective effect against ischemia reperfusion brain injury, the mechanism of protection may be related to inhibiting the injury of free radicals.