Cytotoxicity and Apoptosis Induction of 7- Hydroxycoumarin on SW480 Colon Cancer Cell Line

Introduction: Colorectal cancer is a common disease ranked as the third-highest deathly cancer worldwide. In recent years, for the treatment of cancers scientists have preferred herbal remedies because of their better effects and fewer side effects. So, in this study, the cytotoxicity effect and apoptosis induction of 7-hydroxycycoumarin were investigated on the SW480 colon cancer cell line.Methods: anti-proliferative effect of 7-hydroxycoumarin was investigated using Trypan blue and MTT assay at different concentrations on SW480 cell line. Additionally, apoptosis induction was examined by flow cytometry and real-time PCR.Results: The results show a decrease in viability. IC50 concentrations were observed at the concentrations of 164.2, 80.48, and 41.97 μg/ml for 24, 48, and 72 h, respectively. Of note, IC50 concentrations were not observed in the trypan blue assay. As well, there was a 2.4-fold increase in apoptosis (P<0.001) using Annexin / PI kit by flow cytometry. as well as the increased expression of BAX/BCL2 ratio of about 13.69, which was observed at 100 μg/ml compared to the control.Discussion: The results indicate that 7-hydroxycoumarin could inhibit cell proliferation and induce apoptosis in the SW480 cell line. Therefore, 7-hydroxycycoumarin can be considered as an anti-cancer agent in the complementary treatment of colon cancer.