Study of Broccolini Flavonoids on Anti-cancer Activity in Vitro

To investigate the mechanism of EOF on cell proliferation and apoptosis of human cancer cell line in vitro.The cell growth inhibiting ratio was assessed by the MTT assay,the cell apoptotic was detected with Annexi-V/PI doubled staining flow cytometry(FCM).Human cancer cells line SW480、HepG2、Hela、A549 were treated with different concentrations of EOF respectively.MTT assay showed EOF inhibited the proliferation of human cancer cell line in a dose-dependent manner(P0.01).The MTT assay results showed that the IC50 of EOF for SW480、HepG2 、Hela、A549 1were 88.14、99.65、104.43、79.77 μg/ml respectively.Compared with the control group,the early apoptosis rate in SW480 cells was found to be 12.74%、16.41 %、19.61%、28.28%、50.66%.Experimental results show that the EOF plays a role in tumor therapy by inhibiting proliferation and inducing apoptosis.