Effect on Apoptosis of Human Colon Cancer SW480 Cells by Traditional Chinese Medicine Compound of Zangduqing

Objective:To investigate the inducing apoptosis effect and the underlying mechanism of Zangduqing(ZDQ) on human colon cancer SW480 cells.Method:SW480 cells were cultured with different concentrations of ZDQ(0.05,0.10,0.15,0.20,0.25 g·m L-1) for 12,24,36,48 h in vitro.The cellular cytotoxicity was measured by using the MTT method.Then,SW480 cells were cultured with ZDQ(0.10,0.15,0.20 g·m L-1) for 24 h in vitro and a control group was assigned.The apoptosis was detected by the DAPI and annexin V-FITC / propidium iodide assay.The activities of Caspase-3,-9 were assayed by using Caspase apoptosis detection kit.Protein levels of Bax and Bcl-2 were determined by using Western blot.Result:MTT showed that ZDQ had obvious inhibiting effect on the proliferation of human colon cancer SW480 cells with a dose-and timedependent manner.Compared with the control group,the ZDQ could significantly inhibit the proliferation of SW480 cells,enhance the activity of Caspase-9 and Caspase-3,up-regulate the level of Bax,down-regulate the level of Bcl-2(P 0.01).Conclusion:ZDQ could induce the apoptosis of SW480 cells obviously,which may be mediated by the mitochondrial apoptosis pathway.