2012•农业生物技术：英文版Requires access
Inducing Adventitious Buds from Tomato Callus and Their Rooting
Li-Mei ....................................................................., Zhang
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Abstract
[Objective] The aim was to establish efficient regeneration system of tomato so as to study the genetic transformation of chloroplast in tomato.[Method] The tomato seeds were sterilized and cultured into plantlets.Then,the leaves were cut from plantlets and placed in the MS with 3.0 mg/L 6-BA + 0.3 mg/L IAA to induce callus.Finally,the effect of different hormones and concentrations on induction of adventitious buds from tomato callus and rooting was compared.[Result] The best medium for the induction of differentiation of adventitious buds from callus was:MS + 2.0 mg/L 6-BA + 0.3 mg/L sugar.The best medium for rooting was:1/2MS + 1.0 mg/L IAA.[Conclusion] Appropriate selection of hormone concentrations is the key to establish efficient regeneration system for tomato.