2012•Anhui nongye kexueRequires access
Inducing of Adventitious Buds from Tomato Callus and Rooting
Limei Zhang
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Abstract
[Objective] The aim was to establish efficient regeneration system of tomato so as to prepare for transformation of tomato genetic.[Method] Cultivate tomato seeds processed aseptically to get plantlets.Then,cultivate leaves cut from plantlets in the MS with 3.0 mg/L 6-BA+0.3 mg/L IAA to induce callus.Finally,compare different hormones and concentration on induction of adventitious buds from tomato callus and rooting.[Result] The best medium was MS + 2.0 mg/L 6-BA+0.3 mg/L IAA+30 mg/L sugar,in which many embryo calli were produced;The best medium for rooting was 1/2MS + 1.0 mg/L IAA.[Conclusion] Appropriate choice of hormone concentrations is the key to establish efficient regeneration system for tomato.