ScholarLens Back to search
2003•Journal of Jishou UniversityRequires access

Determination of Cefradin by RP-HPLC

Jiang Jian

Open publisher page 0 citations

Abstract

A RP-HPLC method for the determination of cefradin in raw drug was established.A column of YQG-ODS was used with a mobile phase of V(CH3OH)∶V(4%HAc-NaAc)=3∶7 solution.The UV detector was set at 262 nm.The external standard method was used.There was a good linear correlation in the range of 0.027～0.29 g·L-1,Y(μV·s)=1.88×107X(g·L-1)+20 876,r=0.998 3.The average recovery was 99.7%,RSD was 0.57%(n=3).The elapsed time of a chromatographic analysis was 12 min.The method is rapid,accurate and reliable.

About this research paper

What this paper is about

A RP-HPLC method for the determination of cefradin in raw drug was established.A column of YQG-ODS was used with a mobile phase of V(CH3OH)∶V(4%HAc-NaAc)=3∶7 solution.The UV detector was set at 262 nm.The external standard method was used.There was a good linear correlation in the range of 0.027～0.29 g·L-1,Y(μV·s)=1.88×107X(g·L-1)+20 876,r=0.998 3.The average recovery was 99.7%,RSD was 0.57%(n=3).The elapsed time of a chromatographic analysis was 12 min.The method is rapid,accurate and reliable.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

A RP-HPLC method for the determination of cefradin in raw drug was established.A column of YQG-ODS was used with a mobile phase of V(CH3OH)∶V(4%HAc-NaAc)=3∶7 solution.The UV detector was set at 262 nm.The external standard method was used.There was a good linear correlation in the range of 0.027～0.29 g·L-1,Y(μV·s)=1.88×107X(g·L-1)+20 876,r=0.998 3.The average recovery was 99.7%,RSD was 0.57%(n=3).The elapsed time of a chromatographic analysis was 12 min.The method is rapid,accurate and reliable.

Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Linear correlation, Linear range, Analytical Chemistry (journal), Materials science, Detection limit

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of Cefradin by RP-HPLC — Research Paper | ScholarLens