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2001•Journalof Southwest China Normal UniversityRequires access

Determination of Apomorphini by Resver Phase HPLC

Zhao Mu

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Abstract

A simple,rapid,accurate and reliable resver phase HPLC method was established for the determination apomorphini in its tablets Apomorphini can be separated on Kromasil C 18 (4 6 mm×250 mm,5 μm) column by using methabnol—0 02 mol/LKH 2PO 4(67∶33)(pH=4 60)as mobile phase with UV detector at a wavelength of 272nm The standard curve was linear in the concentration range 10～60 μg/ml and the correlation coefficient was 0 999 7 The average recovery and RSD was 98 56% and 1 44% respectively The detection limit was 4 ng

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What this paper is about

A simple,rapid,accurate and reliable resver phase HPLC method was established for the determination apomorphini in its tablets Apomorphini can be separated on Kromasil C 18 (4 6 mm×250 mm,5 μm) column by using methabnol—0 02 mol/LKH 2PO 4(67∶33)(pH=4 60)as mobile phase with UV detector at a wavelength of 272nm The standard curve was linear in the concentration range 10～60 μg/ml and the correlation coefficient was 0 999 7 The average recovery and RSD was 98 56% and 1 44% respectively The detection limit was 4 ng

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Available abstract

A simple,rapid,accurate and reliable resver phase HPLC method was established for the determination apomorphini in its tablets Apomorphini can be separated on Kromasil C 18 (4 6 mm×250 mm,5 μm) column by using methabnol—0 02 mol/LKH 2PO 4(67∶33)(pH=4 60)as mobile phase with UV detector at a wavelength of 272nm The standard curve was linear in the concentration range 10～60 μg/ml and the correlation coefficient was 0 999 7 The average recovery and RSD was 98 56% and 1 44% respectively The detection limit was 4 ng

Key concepts: Chromatography, High-performance liquid chromatography, Correlation coefficient, Detection limit, Standard curve, Linear correlation, Linear range, Analytical Chemistry (journal)

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