Distribution and drug resistance of pathogens from blood culture

OBJECTIVE To analyze the distribution and drug resistance of the pathogens isolated from the patients with blood infections in the clinical departments and outpatient department from Jan 2006to Jul 2012so as to guide the reasonable clinical use of antibiotics.METHODS 3Dautomated blood culture system was used to culture the blood samples,then the identification of the pathogens and the drug susceptibility testing were performed with the use of VITEK 32auto-MicroScan system,and the data of drug susceptibility testing were statistically analyzed by using WHONET5.4software.RESULTS A total of 335strains of pathogens were isolated from 3718blood specimens with the positive rate of 9.01%,including 170(50.75%)strains of gram-positive bacteria,123(36.72%)strains of gram-negative bacteria,and 39(11.64%)strains of fungi.The pediatrics department(14.03%),hematology department(11.34%),ICU(10.15%),respiratory medicine department(7.76%),and cardiology department(7.16%)ranked the top five departments where the pathogens were isolated.Of 113 strains of coagulase-negative Staphylococcus,totally 85(75.20%)strains of methicillin-resistant coagulasenegative Staphylococcus were detected;of 23strains of Staphylococcus aureus,5(21.70%)strains of methicillinresistance S.aureus were detected.The drug resistance rates of the main gram-positive bacteria to the commonly used antibiotics such as penicillin,oxacillin,erythromycin,and clindamycin were higher than 50.00%,and all the strains were susceptible to vancomycin,linezolid,and quinupristin-dalfopristin;the drug resistance rate of the Enterococci to levofloxacin was 78.3%,erythromycin 87%,gentamicin 60.8%.CONCLUSION The coagulasenegative Staphylococcus is the predominant species of pathogens isolated from the blood cultures and is high drug resistant,it is necessary to perform the blood cultures so as to reasonably use antibiotics.