Drug resistance and distribution of pathogens isolated from blood culture

OBJECTIVE To investigate the distribution and drug resistance of pathogens isolated from positive blood culture in a hospital of the northern Zhejiang so as to provide evidence for rational use of antibiotics in clinic.METHODS The blood samples were cultured by Bact/Alert120 system,the positive samples were inoculated into blood agar,microorganisms were identified by VITEK-2 Compact,then the drug susceptibility testing was performed by using the K-B method,and the result was determined according to CLIS 2009standard;WHONET 5.4 software was used to analyze the data.RESULTS Of totally 7941 blood culture specimens,there were 588 strains of pathogens isolated with the positive rate of 7.40%,including 376(64.0%)strains of gram-positive bacteria,164(27.9%)strains of gram-negative bacteria,and 22(3.7%)strains of fungi;the coagulase-negative Staphylococcus(CNS),Klebsiella pneumoniae,Staphylococcus aureus,and Escherichia coli ranked the top four species of pathogens,accounting for 50.7%,9.9%,9.5%,and 8.5%,respectively.The CNS,S.aureus,and Enterococcus faecalis have produced certain drug resistance to all the antibiotics except vancomycin and linezolid with the drug susceptibility rate of 100.0%,the MRSA strains accounted for 57.15%,the MRCNS accounted for 41.6%;the E.coli and K.pneumoniae were highly resistant to all the antibiotics except imipenem,meropenem,and amikacin;all the Candida albicans and Candida tropicalis were sensitive to amphotericin B,5-fluorocytosine,voriconazole and itraconazole except one strain of C.albicans resistant to amphotericin B.CONCLUSION The detection rate of the pathogens isolated from blood culture is relatively low,the gram-positive bacteria are predominant,the CNS strains occupy a larger proportion,the drug resistance rate is relatively high.It is of great significance to reduce the incidence of nosocomial infections and guide the reasonable use of antibiotics through understanding the distribution and drug resistance of the pathogens causing bloodstream infections.