Distribution and drug resistance of pathogens from blood culture specimens

OBJECTIVE To investigate the distribution and the drug resistance of the pathogens from blood culture specimens in 2011 so as to guide the clinical rational use of antibiotics.METHODS ATB Expression automatic microbiological analysis system was used to identify gram-positive pathogens and fungi,meanwhile the in vitro drug susceptibility testing of fungi was performed,then VITEK 60 automatic microbiological analysis system was employed to identify the gram-negative pathogens,the drug susceptibility testings were performed for the grampositive and gram-negative pahogens.The distribution of the isolated pathogens and the results of drug susceptibility testings were statistically analyzed by using WHONET 5.6.RESULTS In these 320 samples,there were 198 strain of gram-positive cocci (61.9%) and 111 strains of gram-negative bacilli (34.7%).The most commons species of the gram-positive were coagulase-negative Staphylococci,Enterococci and Staphylococcus aureus,and no strains of vancomycin-resistant strains were found,with the drug susceptibility rates of 100.0%.The coagulase-negative Staphylococci were highly sensitive to linezolid and nitrofurantoin,however the strains varied in the drug resistance to other antibiotics.All of the fungi were sensitive to amphotericin B,flurocytosine,fluconazole,and itraccnazole,and no drug resistant strains were detected.CONCLUSION The pathogens isolated from blood specimens are widely distributed and vary significantly in the drug resistance.It is necessary to monitor the isolates from blood culture in early stage and analyze the drug resistance,which can provide important evidence for the clinicians to rationally use antibiotics and to prevent the abuse of antibiotics and the emergence of new drugresistant isolates.