Distribution and drug resistance of pathogens isolated from 1368 blood culture specimens

OBJECTIVE To investigate the distribution and drug resistance of pathogens from blood infection samples,so as to provide evidence for rational use of antibiotics in clinic.METHODS BACTEC9050 automated blood culture system manufactured by American BD Company and its corresponding adult aerobic resin bottles were used.All pathogens were identified in accordance with routine operating procedures.Drug susceptibility test was performed by K-B diffusion method and the results were analyzed by WHONET 5.5 software.RESULTS Totally 119 strains of 29 species of pathogens were isolated from 1368 blood culture samples,with the positive rate of 8.7%.Among these pathogens,63 isolates(52.9%) were gram-positive cocci,51 isolates(42.9%) were Gram-negative bacilli,4 isolates(3.3%) were fungi,1 isolate(0.8%) was gram-positive bacilli.Drug resistance rates of Staphylococcus,Streptococcus and Enterococcus were different,but there were no vancomycin-resistant strains detected.The species of gram-negative bacilli had certain degree of resistance to antibiotics,but no strains resistant to imipenem(IPM) or meropenem(MEM)were found;Candida albicans was resistant to most antifungal agents,but all the resistance rate was less than 25.0%,while the isolates were 100.0% sensitive to amphotericin B.CONCLUSION The spectrum of blood culture pathogens is wide,and the predominant pathogens are gram-positive cocci,followed by gram-negative bacilli.The drug resistance is prevalent,so the monitoring of drug resistance of blood culture pathogens should be carried out in a timely manner to guide rational use of antibiotics.