Distribution and drug susceptibility analysis of blood culture-derived pathogens from patients in ICU

OBJECTIVE To understand the distribution and drug resistance of pathogens from blood culture specimens of ICU patients and guide clinical rational use of antibiotics.METHODS The VITEK32 automatic microbiological analysis system was used to identify bacteria;VITEK32 automatic microbiological analysis system and Kirby-Bauer agar diffusion method were used to test in vitro drug susceptibility;WHONET5.5 software was applied to analyze the distribution of pathogenic bacteria isolated and the results of drug susceptibility tests.RESULTS Blood culture was performed for a total of 1632 cases and 237 cases were positive.256 strains of pathogens were isolated.The positive rate was 14.5%.The isolated pathogens included 110(43.0%)strains of gram-positive bacteria,86(33.6%) strains of gram-negative bacteria,and 60(23.4%) strains of fungi.Staphylococcus haemolytic and S.hominis were the main gram-positive bacteria and mainly susceptible to vancomycin with the drug susceptibility rate of 100.0%.gram-negative bacteria isolates except Burkholderia cepacia and Klebsiella pneumonia were mainly susceptible to imipenem and cefoperazone/sulbaotam.Candida albicans was the major fungi and sensitive to common antifungal agents.CONCLUSION The pathogens isolated from blood specimens in ICU are widely distributed and have great differences in the drug resistance.It is necessary to early monitor the isolates from blood culture and analyze the drug resistance,which can provide important evidence for clinicians to rationally use antibiotics and to prevent the abuse of antibiotics and new drug-resistant isolates.