Induction Effect of Cadmium on Peripheral Erythrocyte Micronuclei in Carassius auratus and Oriental weatherfish
Hongzhao Lu
Abstract
Hongzhao Lu
Abstract
[Objective] The study aimed to the effects of various Cd2+ concentrations and exposure time on erythrocyte micronuclei in peripheral erythrocyte micronuclei in Carassius auratus and Oriental weatherfish.[Method]Using static water culture, Carassius auratus and Oriental weatherfish were exposed to solutions containing Cd2+ with mass concentrations of 0.08, 1.72 and 6.84 mg/L, and blood was collected from tail veins to make smears which were observed under a oil lens afterwards. [Result]In the initial exposure period, Carassius auratus and Oriental weatherfish were unwell obviously, being afraid and unpeaceful and escaping everywhere. With the increase in Cd2+ concentration, this phenomenon became more distinct. In addition, as the prolonging of exposure time, fishes moved slowly, powerlessly and less and reacted slowly, and moe and more excrement deposited at the bottom of aquariums. [Conclusion] Cd2+ could obviously induce Carassius auratus and Oriental weatherfish to generate micronuclei, showing certain dose-effect and time-effect relation under certain conditions.
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[Objective] The study aimed to the effects of various Cd2+ concentrations and exposure time on erythrocyte micronuclei in peripheral erythrocyte micronuclei in Carassius auratus and Oriental weatherfish.[Method]Using static water culture, Carassius auratus and Oriental weatherfish were exposed to solutions containing Cd2+ with mass concentrations of 0.08, 1.72 and 6.84 mg/L, and blood was collected from tail veins to make smears which were observed under a oil lens afterwards. [Result]In the initial exposure period, Carassius auratus and Oriental weatherfish were unwell obviously, being afraid and unpeaceful and escaping everywhere. With the increase in Cd2+ concentration, this phenomenon became more distinct. In addition, as the prolonging of exposure time, fishes moved slowly, powerlessly and less and reacted slowly, and moe and more excrement deposited at the bottom of aquariums. [Conclusion] Cd2+ could obviously induce Carassius auratus and Oriental weatherfish to generate micronuclei, showing certain dose-effect and time-effect relation under certain conditions.
Key concepts: Carassius auratus, Micronucleus test, Cadmium, Toxicology, CADMIUM EXPOSURE, Biology, Chemistry, Toxicity