Association of coronary heart disease with two polymorphisms within C reactive protein gene promoter region in ethnic Han Chinese

Objective To investigate the distribution of two single nucleotide polymorphism(SNP)-717A/G and-390C/A/T-within the C reactive protein(CRP)promoter region in ethnic Han Chinese and the association with coronary heart disease(CHD).Methods Altogether positive CHD patients and negative controls were studies in this study.The two target fragments were amplied by PCR.Then the-717A/G polymorphism was screened by restriction fragment length polymorphism(RFLP)and the-390C/A/T polymorphism by Sequence Specific PCR(SSP).At last,the association was studied by proper statistical analysis.Results There was no significant difference in the distribution of-717A/G polymorphism betweenpatients and controls.However,the frequencies of allels in-390C/A/T polymorphismwere significant different between them(P0.05),and the genotype frequencies were also different(P0.05).Conclusion There is no association between-717A/G polymorphism and CHD,however,-309C/A/T polymorphism is associated with CHD,and the T allele in this polymorphism maybe an independent risk factor of CHD.