2013•Unpublished venueRequires access

Determination of eight ginsenosides in Renshenshouwu capsules by HPLC-DAD

Ji Lin

Abstract

Objective: To establish an HPLC-DAD method for simultaneous determination of eight ginsenosides in Renshenshouwu capsules.Method: Ultimate C 18 column(4.6 mm × 250 mm,5 μm) was adopted for gradient elution,with acetonitrile and water as the mobile phase.The flow rate was 1 mL·min-1,the column temperature was set at 30 ℃,and the detection wavelength was 203 nm.Result: A good linearity was observed in the range of 0.242-12.1,0.222-11.1,0.251-25.1,0.245-24.5,0.23223.2,0.232-23.2,0.264-26.4,0.244-24.4 μg for ginsenoside Rg 1,Re,Rb 1,Rc,Rb 2,Rb 3,Rd and 20(S)-ginsenoside Rg 3,respectively,with the average recoveries of 102.7%,103.2%,101.6%,101.2%,102.0%,100.7%,101.9%,102.0%,respectively.Conclusion: The method was so simple,accurate and effective that it could be used for quality control of the above eight components in Renshenshouwu capsules.

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Key concepts: Chromatography, Chemistry, Ginsenoside, Gradient elution, High-performance liquid chromatography, Acetonitrile, Ginsenoside Rg1, Analytical Chemistry (journal)