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HPLC determination of ginsenoside Rb_1 in Shuangshenlong capsules

Xiaoping Han

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Abstract

Objective:To develop a method for determination of ginsenoside Rb_1 in Shuangshenlong capsules by HPLC.Method:The column was Kromasil C_(18)(KR100-5 C_(18),4.6mm×250 mm,5μm).Acetonitrile-water (28.7:71.3)was used as the mobile plase,the flow rate was 1.0 mL·min~(-1)and detection wavelength was 203 nm.Results:The linear range was between 0.848-4.24 μg for ginsenoside Rb_1(r=0.9991).The mean recovery was 99.3%,RSD=1.2%.Conclusion:This method is found to be sensitive,quick and accurate for the determina- tion of ginsenoside Rb_1 in Shuangshenlong capsules.

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What this paper is about

Objective:To develop a method for determination of ginsenoside Rb_1 in Shuangshenlong capsules by HPLC.Method:The column was Kromasil C_(18)(KR100-5 C_(18),4.6mm×250 mm,5μm).Acetonitrile-water (28.7:71.3)was used as the mobile plase,the flow rate was 1.0 mL·min~(-1)and detection wavelength was 203 nm.Results:The linear range was between 0.848-4.24 μg for ginsenoside Rb_1(r=0.9991).The mean recovery was 99.3%,RSD=1.2%.Conclusion:This method is found to be sensitive,quick and accurate for the determina- tion of ginsenoside Rb_1 in Shuangshenlong capsules.

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Available abstract

Objective:To develop a method for determination of ginsenoside Rb_1 in Shuangshenlong capsules by HPLC.Method:The column was Kromasil C_(18)(KR100-5 C_(18),4.6mm×250 mm,5μm).Acetonitrile-water (28.7:71.3)was used as the mobile plase,the flow rate was 1.0 mL·min~(-1)and detection wavelength was 203 nm.Results:The linear range was between 0.848-4.24 μg for ginsenoside Rb_1(r=0.9991).The mean recovery was 99.3%,RSD=1.2%.Conclusion:This method is found to be sensitive,quick and accurate for the determina- tion of ginsenoside Rb_1 in Shuangshenlong capsules.

Key concepts: Chemistry, Chromatography, Ginsenoside, High-performance liquid chromatography, Ginsenoside Rg1, Linear range, Acetonitrile, Detection limit

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