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2013•China Journal of Chinese Materia MedicaRequires access

Determination of eight ginsenosides in Renshenshouwu capsules by HPLC-DAD

Li-Na Ji, Wei-Hong Feng, Zhimin Wang, Jing-Jing Zhu, Qi-Wei Zhang, Liang‐Mian Chen, Chun Li

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Abstract

OBJECTIVE: To establish an HPLC-DAD method for simultaneous determination of eight ginsenosides in Renshenshouwu capsules. METHOD: Ultimate C18 column (4.6 mm x 250 mm, 5 microm) was adopted for gradient elution, with acetonitrile and water as the mobile phase. The flow rate was 1 mL x min(-1), the column temperature was set at 30 degrees C, and the detection wavelength was 203 nm. RESULT: A good linearity was observed in the range of 0.242-12.1, 0.222-11.1, 0.251-25.1, 0. 245-24.5, 0.232-23.2, 0.232-23.2, 0.264-26.4, 0.244-24.4 microg for ginsenoside Rg1, Re, Rb1, Rc, Rb2, Rb3, Rd and 20(S)-ginsenoside Rg3, respectively, with the average recoveries of 102.7%, 103.2%, 101.6%, 101.2%, 102.0%, 100.7%, 101.9%, 102.0%, respectively. CONCLUSION: The method was so simple, accurate and effective that it could be used for quality control of the above eight components in Renshenshouwu capsules.

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What this paper is about

OBJECTIVE: To establish an HPLC-DAD method for simultaneous determination of eight ginsenosides in Renshenshouwu capsules. METHOD: Ultimate C18 column (4.6 mm x 250 mm, 5 microm) was adopted for gradient elution, with acetonitrile and water as the mobile phase. The flow rate was 1 mL x min(-1), the column temperature was set at 30 degrees C, and the detection wavelength was 203 nm. RESULT: A good linearity was observed in the range of 0.242-12.1, 0.222-11.1, 0.251-25.1, 0. 245-24.5, 0.232-23.2, 0.232-23.2, 0.264-26.4, 0.244-24.4 microg for ginsenoside Rg1, Re, Rb1, Rc, Rb2, Rb3, Rd and 20(S)-ginsenoside Rg3, respectively, with the average recoveries of 102.7%, 103.2%, 101.6%, 101.2%, 102.0%, 100.7%, 101.9%, 102.0%, respectively. CONCLUSION: The method was so simple, accurate and effective that it could be used for quality control of the above eight components in Renshenshouwu capsules.

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Available abstract

OBJECTIVE: To establish an HPLC-DAD method for simultaneous determination of eight ginsenosides in Renshenshouwu capsules. METHOD: Ultimate C18 column (4.6 mm x 250 mm, 5 microm) was adopted for gradient elution, with acetonitrile and water as the mobile phase. The flow rate was 1 mL x min(-1), the column temperature was set at 30 degrees C, and the detection wavelength was 203 nm. RESULT: A good linearity was observed in the range of 0.242-12.1, 0.222-11.1, 0.251-25.1, 0. 245-24.5, 0.232-23.2, 0.232-23.2, 0.264-26.4, 0.244-24.4 microg for ginsenoside Rg1, Re, Rb1, Rc, Rb2, Rb3, Rd and 20(S)-ginsenoside Rg3, respectively, with the average recoveries of 102.7%, 103.2%, 101.6%, 101.2%, 102.0%, 100.7%, 101.9%, 102.0%, respectively. CONCLUSION: The method was so simple, accurate and effective that it could be used for quality control of the above eight components in Renshenshouwu capsules.

Key concepts: Chromatography, Chemistry, Ginsenoside Rg1, Ginsenoside, High-performance liquid chromatography, Gradient elution, Acetonitrile, Analytical Chemistry (journal)

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