Physiobiochemical Characteristics and Stamen Development Characteristics of LRCMS Flower in Winter Rapeseed(Brassica campestris)

The LRCMS is a cytoplasmic male sterile material selected from the off springs of‘Longyou 8'× ‘Longyou 9'.POD,SOD,CAT activities and soluble protein content of LRCMS in flowering buds and flowers,peroxidase(POD)isozyme changes during the abortion process were investigated.The results showed that:(1)POD activity of all the tested materials in flower is generally higher than that of buds.(2) Sterile material POD activity is higher than that of maintainer line.Compared with the maintainer line,the increase of POD activity in kale sterile material is greater than that of LRCMS and‘Longyou 9'.POD ac-tivities in LRCMS flowers and buds are 15.0%and 9.0%higher than that of the maintainer line.CAT activity in sterile material flowers is higher than that of maintainer line(89%~293%higher),while CAT activity in buds is lower than that of the maintainer line(25%~43% lower);and there is no significant difference between LRCMS and other sterile materials.SOD activity in sterile material flowers is lower than that of the maintainer line,while SOD activity in buds is higher than that of the maintainer line(6%~ 112%higher),and the increase in LRCMS is lower than that of other sterile materials.Flowers SOD activity is 15%~25%lower than that of the maintainer line and there is no significant difference between LRCMS and other sterile materials.Buds of the maintainer line and their protein content is 0.014~0.020 mg/g,while the protein content in buds of the sterile material is 0.012~0.016mg/g.Protein contents of all sterile materials are lower than that of the maintainer line.(3)POD isoenzyme test indicated an Rfvalue of 0.623band in flower of LRCMS which is missing in other male sterile lines and fertile line‘Longyou 9'.(4)LRCMS sterile stamens starting at the sporogenous cell,sporogenous cell adhesion,nuclear-free kernel,tapetum absence.The study find that LRCMS and other CMS have different genetic backgrounds.It is a new type of infertility.