Activities of Protective Enzymes of Reactive Oxygen Metabolism during Aborting Process of 5 Kinds of Cytoplasmic Male Sterile Wheat Lines

Male sterility is of significant value in wheat heterosis utilization.For understanding the changing characteristics of protective enzymes of reactive oxygen metabolism during the process of pollen abortion in alloplasmic male sterile wheat lines,the activities of peroxidase(POD),catalase(CAT),and superoxide dismutase(SOD)in anther were detected in 5kinds of cytoplasmic male sterile line and the maintainer lines at different developmental stages.Results showed that the activity of POD in sterile anther was higher than that in fertile anther since early uninucleate stage.The general trend of CAT activity in all sterile lines was decreasing,while that in the fertile line was increasing since later uninucleate stage.The general trend of SOD activity was increasing in all anthers,and the enzyme activity of sterile lines was higher than that of maintainer lines.The difference on the activities of POD,CAT,SOD between the 5kinds of sterile wheat lines and their maintainer lines was analyzed,it showed that the abortion stage of U116 A,Ju116A,Va116 Aand C6116A was at the late uninucleate stage,and that of K116 A was from the late uninucleate to binucleate.It was suggested that the abortion stage was different among the sterile lines,and male sterility may be related with the activities of POD,CAT,SOD.