2003•Journal of the South China Agricultural UniversityRequires access
Isoenzyme Analysis on Flower Bud of Male Sterile Line and Its Maintainer in Broccoli
Li You
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Abstract
By means of vertical slab polyacrylamide gel electrophoresis, isoenzymes of peroxidase (POD), superoxide dismutase (SOD), polyphenobxide (PPO) and catechol oxidase (CAO) in the calyx, petal, stamen and pistil of broccoli flower buds male sterile line and its maintainer were analyzed. Results showed that isoenzyme pattern differences of the four enzymes existed not only between male sterile line and its maintainer, but also among calyx, petal, stamen and pistil within the male sterile line and maintainer. The POD, CAO isoenzymes showed more isoenzyme bands in the male sterile line than that in its maintainer, but PPO, SOD isoenzymes less isoenzyme bands in the male sterile line than that in its maintainer. The greatest difference in the four isoenzymes between the male sterile line and its maintainer was seen in the stamen.