2006•Zhonghua xueyexue zazhi/Zhōnghuá xuèyèxué zázhìRequires access

Adriamycin enhances anti-human DR5 monoclonal antibody (mDRA-6) induced HL-60 cells apoptosis

LU Fen

Abstract

Objective To investigate synergistic killing effect of anti-human DR5(death receptor 5 of TRAIL)monoclonal antibody(mDRA-6)and adriamycin(Adr)on HL-60 cells.Methods mDRA-6 was prepared by immunizing BALB/c mice with DR5 protein.DR5 expression on Adr-treated HL-60 cells was de- tected by flow cytometry.Morphologic changes of HL-60 cells were observed under fluorencence microscope. Cytotoxic and apoptotic effects of mDRA-6 and Adr on HL-60 cells were measured by MTT analysis.DNA fragmentation was detected by agarose gel electrophoresis.Results Adr induce DR5 expression on HL-60 cells.Cell budding,chromatin condensation and apoptotic body formation were observed in HL-60 cells trea- led by mDRA-6 and Adr,Death and apoptosis of these cells and DNA ladder were exhibited on agarose gel e- lectrophoresis.Conclusion mDRA-6 and Adr have synergistic killing effect on HL-60 cells.

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Key concepts: Chemistry, Apoptosis, Agarose gel electrophoresis, Molecular biology, Flow cytometry, Cytotoxic T cell, Monoclonal antibody, Fragmentation (computing)