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Synergistic effect of anti-human DR5(death receptor 5)monoclonal antibody(mDRA-6)and Cisplatin on HL-60 cells

Shu Li

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Abstract

Objective:To study the synergistic induction of apoptosis by the combination of anti-human DR5(death receptor 5)monoclonal antibody(mDRA-6)and cisplatin(DDP)on HL-60 cells.Methods:To produce anti-human DR5 monoclonal antibody(mDRA-6)by immunizing BALB/c mice using DR5 protein.The change of DR5 expression after DDP-treated on HL-60 cells was detected by FACS.Morphologic changes of HL-60 cells were observed under fluorencence microscope.Cytotoxic and apoptotic effects of mDRA-6 and DDP on HL-60 cells were measured by MTT analysis.DNA fragmentation was detected by agarose gel electrophoresis.Results:DDP could induce DR5 expression on HL-60 cells.Chromatin condensation,budding and apoptotic bodies were observed in HL-60 cells treated by the combination of mDRA-6 and DDP;Death and apoptosis of HL-60 cells treated by the combination of mDRA-6 and DDP were increased obviously.DNA ladder in the combination of mDRA-6 and DDP-treated HL-60 cells was exhibited obviously on agarose gel electrophoresis.Conclusion:Anti-human DR5 monoclonal antibody(mDRA-6)and DDP could synergistic induce apoptosis of HL-60 cells.

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What this paper is about

Objective:To study the synergistic induction of apoptosis by the combination of anti-human DR5(death receptor 5)monoclonal antibody(mDRA-6)and cisplatin(DDP)on HL-60 cells.Methods:To produce anti-human DR5 monoclonal antibody(mDRA-6)by immunizing BALB/c mice using DR5 protein.The change of DR5 expression after DDP-treated on HL-60 cells was detected by FACS.Morphologic changes of HL-60 cells were observed under fluorencence microscope.Cytotoxic and apoptotic effects of mDRA-6 and DDP on HL-60 cells were measured by MTT analysis.DNA fragmentation was detected by agarose gel electrophoresis.Results:DDP could induce DR5 expression on HL-60 cells.Chromatin condensation,budding and apoptotic bodies were observed in HL-60 cells treated by the combination of mDRA-6 and DDP;Death and apoptosis of HL-60 cells treated by the combination of mDRA-6 and DDP were increased obviously.DNA ladder in the combination of mDRA-6 and DDP-treated HL-60 cells was exhibited obviously on agarose gel electrophoresis.Conclusion:Anti-human DR5 monoclonal antibody(mDRA-6)and DDP could synergistic induce apoptosis of HL-60 cells.

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Available abstract

Objective:To study the synergistic induction of apoptosis by the combination of anti-human DR5(death receptor 5)monoclonal antibody(mDRA-6)and cisplatin(DDP)on HL-60 cells.Methods:To produce anti-human DR5 monoclonal antibody(mDRA-6)by immunizing BALB/c mice using DR5 protein.The change of DR5 expression after DDP-treated on HL-60 cells was detected by FACS.Morphologic changes of HL-60 cells were observed under fluorencence microscope.Cytotoxic and apoptotic effects of mDRA-6 and DDP on HL-60 cells were measured by MTT analysis.DNA fragmentation was detected by agarose gel electrophoresis.Results:DDP could induce DR5 expression on HL-60 cells.Chromatin condensation,budding and apoptotic bodies were observed in HL-60 cells treated by the combination of mDRA-6 and DDP;Death and apoptosis of HL-60 cells treated by the combination of mDRA-6 and DDP were increased obviously.DNA ladder in the combination of mDRA-6 and DDP-treated HL-60 cells was exhibited obviously on agarose gel electrophoresis.Conclusion:Anti-human DR5 monoclonal antibody(mDRA-6)and DDP could synergistic induce apoptosis of HL-60 cells.

Key concepts: Chemistry, Apoptosis, Monoclonal antibody, Molecular biology, Antibody, Cisplatin, Cytotoxic T cell, Agarose gel electrophoresis

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Synergistic effect of anti-human DR5(death receptor 5)monoclonal antibody(mDRA-6)and Cisplatin on HL-60 cells — Research Paper | ScholarLens