2006•PubMedRequires access
[Effect of anti-human DR5 monoclonal antibody on the apoptosis of human hepatocyte HL7702 cell lines].
Jun Zhang, Yuanfang Ma, Guangchao Liu, Shulian Li, Feng Lü
Open publisher page 1 citations
Abstract
AIM: To evaluate the effect of a novel anti-human DR5 monoclonal antibody (mAb mDRA-6) on the apoptosis of human hepatocyte HL7702 cell lines. METHODS: DR5 expression on HL7702 cell surface was determined by flow cytometry(FCM). The effect of mAb mDRA-6 on the morphous of HL7702 cells was observed by fluorescence microscope. mDRA-6 cytotoxicity on HL7702 cells was detected by using MTT analysis. The rate of apoptosis was detected by FCM with Annexin V-FITC/PI staining. RESULTS: HL7702 cells treated with mDRA-6 exhibited some typical apoptotic features in morphology. MTT analysis showed the death rate of HL7702 cells was 39% in the presence of 40 mg/L mDRA-6 for 6 hours. FCM detection indicated the apoptosis rate of the cells was 25.5% in the presence of 3 mg/L mDRA-6 for 6 hours with Annexin V-FITC/PI staining. CONCLUSION: mDRA-6 can induce the apoptosis of HL7702 cells.