Determination of eight components in Qingrejiedu granules by HPLC with UV switch

Objective: To develop an HPLC method for simultaneous determination of chlorogenic acid,geniposide,cynaroside,phillyrin,baicalin,luteolin,baicalein and wogonin in Qingrejiedu granules. Methods: The chromatographic separation was carried out on a Phenomenex Luna 5μ-C18column( 4. 6 mm × 250 mm,5 μm) with a mobile phase consisting of methanol( A)- 0. 1% phosphoric acid solution( B) in a gradient mode at a flow rate of 1 m L·min- 1. The UV detection wavelength was set at 326 nm for chlorogenic acid in the first 26 min,238 nm for geniposide during 26- 35 min,350 nm for cynaroside during 35- 44 min,230 nm for phillyrin during 44- 48 min,276 nm for baicalin during 48- 53 min,350 nm for luteolin during 53- 60 min,276 nm for baicalein and wogonin between60 and 85 min. Results: Excellent chromatographic separation was achieved and the ranges for linear correlation of chlorogenic acid,geniposide,cynaroside,phillyrin,baicalin,luteolin,baicalein and wogonin were 0. 041 3- 0. 413μg( r = 0. 999 6),0. 034 0- 0. 340 μg( r = 0. 999 9),0. 037 1- 0. 371 μg( r = 0. 999 5),0. 026 4- 0. 264 μg( r =0. 999 3),0. 196 0- 1. 960 μg( r = 0. 999 8),0. 010 8- 0. 108 μg( r = 0. 999 2),0. 021 2- 0. 212 μg( r = 0. 999 2)and 0. 005 7- 0. 057 μg( r = 0. 999 0),respectively. The average recoveries( n = 6) of the eight components were98. 4%( RSD = 1. 6%),99. 2%( RSD = 1. 4%),98. 5%( RSD = 1. 7%),100. 7%( RSD = 2. 0%),99. 0%( RSD = 0. 9%),98. 8%( RSD = 1. 9%),98. 1%( RSD = 1. 8%) and 99. 9%( RSD = 1. 3%). The eight ingredient contents in 3 batches of samples were: 0. 326- 0. 775 mg·g- 1( chlorogenic acid),0. 167- 0. 581 mg·g- 1( geniposide),0. 093- 0. 216 mg·g- 1( cynaroside),0. 107- 0. 304 mg·g- 1( phillyrin),1. 574- 3. 810 mg·g- 1( baicalin),0. 092- 0. 114 mg·g- 1( luteolin),0. 175- 0. 625 mg·g- 1( baicalein) and 0. 042- 0. 188 mg·g- 1( wogonin) by HPLC detection. Conclusion: The established method is simple,rapid,sensitive and accurate,which can be applied to determine chlorogenic acid,geniposide,cynaroside,phillyrin,baicalin,luteolin,baicalein and wogonin in Qingrejiedu granules.