Simultaneous Determination of Six Effective Components in Qingre Jiedu Capsules by HPLC

Objective: A HPLC method was established for the determination of chlorogenic acid,geniposide,luteolin,baicalin,phillyrin and wogonin in Qingrejiedu capsules. Method: Eclipse XDB column( 4. 6 mm × 150 mm,5 μm) was adopted and eluted in gradient with the mobile phase of acetonitrile-0. 4%phosphoric acid solution. The flow rate was 0. 80 m L ·min- 1. The detection wavelength was set at 275 nm.Chlorogenic acid, geniposide, luteolin, baicalin, phillyrin and wogonin could be completely separated from adjacent mass spectra peaks. Result: The HPLC method showed a good linear range of 7. 83-313 mg·L- 1( r =0. 999 9) for chlorogenic acid,4. 30-132 mg·L- 1( r = 0. 999 8) for geniposide,0. 836-33. 5 mg·L- 1( r =0. 999 7) for luteolin,4. 36-349 mg·L- 1( r = 1. 000 0) for baicalin,0. 731-58. 4 mg·L- 1( r = 0. 999 7) for phillyrin and 0. 314-12. 6 mg·L- 1( r = 0. 999 7) for wogonin,with the recoveries above 98%. Conclusion: The method is simple,accurate and highly reproducible and so can be used to remove interferences from other components and evaluate the quality control of the preparation.