Simultaneous Determination of Chlorogenic Acid,Forsythin,Baicalin,Wogonin and Apigenin in Compound Jinyinhua Granules by HPLC

Objective: A rapid and sensitive method for HPLC was established to simultaneouly determine chlorogenic acid,forsythin,baicalin,wogonin and apigenin. Method: Chromatographic separation was carried out on Agilent Eclipse XDB-C18column( 4. 6 mm × 250 mm,5 μm) under gradient elution( 0-0. 01 min,A phase is 25%; 0. 01-10 min,A phase is 25%-53%; 10-16 min,A phase is 53%-78%) with methanol( A phase)-water/0. 2% phosphate( B phase) as mobile phase at a flow rate of 1.00 mL·min- 1to elute at 25℃. The injection volume was 20 μL and the detection was adopted UV detector at 278 nm. Result: These five components were seperated basically within 25 minutes with good linearity. The regression equations were Y =2 350. 4X- 137. 27,r = 0. 999 6( for chlorogenic acid),Y = 1 161. 7X + 18. 474,r = 0. 999 6( for forsythin),Y = 3 204. 2X- 423. 45,r = 0. 998 8( for baicalin),Y = 4 792. 7X- 93. 15,r = 0. 998 5( for wogonin),Y =1 434. 1X- 8. 413 2,r = 0. 999 3( for apigenin). The average recoveries were: chlorogenic acid 99. 47%( RSD 0. 8%),forsythin 98.26%( RSD 0. 9%),baicalin 100.4%( RSD 1.9%),wogonin 99.29%( RSD1. 1%),apigenin 98. 79%( RSD 1. 1%). Conclusion: The HPLC method has good accuracy,reliability,and repeatbility. It is suitable for compound Honeysuckle granules and traditonal Chinese medicines containing the same ingredients to improve their quality control.