2010•Unpublished venueRequires access
Cluster buds induction and its regeneration system in Smilax riparia A. DC.
Weihua Yu, Ping Meng, Zhang XiangJun, Pan YingNan, Chen Shao-zhen, Sumei Huang, Tang Jun
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Abstract
The shoot tip of wild Smilax riparia A.DC was used as explants to conduct experiment on the induction,multiplication and rooting of cluster buds in media with different combinations of 6-BA(0.5-5.0 mg/L) and NAA(0.1-1.0 mg/L).The results showed that the optimum medium for cluster buds induction was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+ 0.3% activated charcoal.Browning of explants was completely controlled in this medium and the cluster buds grew earlier with 75.0 % induction rate.The optimum medium for multiplication of cluster buds was MS+6-BA 0.5 mg/L+NAA 0.1 mg/ L with 4.2 multiplication coefficient.The optimum medium for rooting was 1/2MS supplemented with 1.0 mg/L NAA,the rooting rate reached up to 87.0%,the number of root was from 4 to 7 and the average root length reached up to 1.2 cm.The rooting plantlets were found able to survive in the transplantation medium(nutrition soil ∶ sawdust ∶ yellow mud= 1∶1∶2) with 80.0% of transplant survival rate.