Tissue culture of medicinal plant Millettia speciosa Champ.

The young stems of Millettia speciosa Champ.were used as explants.After sterilization with 0.1% HgCl2 for 12,15 and 18 min,the callus and buds of explants were induced in MS medium added with different concentrations of 6-BA(2.0,2.5 mg/L)and NAA(0.2,1.0 mg/L).The 1/2MS medium added with NAA(0-2.5 mg/L) and IBA(0-2.5 mg/L) was used to conduct root culture in induced plantlets.The results showed that the browning and infection rate of explants were lower when sterilized for 15 min by 0.1% HgCl2.The callus of explants could be induced in medium added with different combinations of 6-BA and NAA,but the induction rate of callus was the highest in medium added with 1.0 mg/L NAA.The axillary buds in stem could be germinated in media added with 2.0 and 2.5 mg/L 6-BA,but the highest germination rate only reached 78.6%.The best medium for multiplication of regenerated axillary buds was MS+2.0 mg/L 6-BA+ 0.05 mg/L NAA,the multiplication coefficient was 4.0-5.0 after cultured for 30 days.It was found that the rooting rate of multiplied buds was up to 66.7% in medium 1/2MS+NAA 2.5 mg/L+IBA 2.5 mg/L,and the survival rate of transplants reached 85% on 30 days after transplanted to mixed matrix of vegatable soil and river sand(3∶1).