Tissue culture and rapid propagation technology for pineapple variety Tainong 17

The crown bud of pineapple variety Tainong 17 was used as explants,different hormone combinations ranging from 1.0-4.0 mg/L 6-BA,0.1-0.5 mg/L NAA and 1.0-4.0 mg/L IBA were added to MS or 1/2MS medium to conduct the induction of bud,subculture of cluster buds and rooting of plantlets.The results showed that MS+6-BA(3.0 mg/L)+ NAA(0.1 mg/L) was the best medium for the induction of crown buds,and subculture of the cluster buds with 6.8 of multiplication coefficient in period of 22 days.The optimal medium for rooting was 1/2MS + IBA(3.0 mg/L)+ NAA(0.5 mg/L),and the mixture of sand and pond sludge(1∶2) was found to be the best temporary planting medium for rooted plantlets with 94% of survival rate,and the plantlets grew well and vigorouly.