Culture in vitro and rapid propagation techniques of buds and leafs in Camellia oleifera

The effects of plant growth regulators on the adventitious bud proliferation and rooting in Camellia oleifera were studied by using new shoot(with buds) and leaf as explants to establish a fast reproductive system.And the effects of different medium on survival rate of transplanted tissue culture seedlings were also investigated.The results showed that:(1) The highest frequency of shoot formation was obtained when explants(stem with buds) were cultured on 1/2MS+NAA(0.5 mg/L) +6-BA(1.0 mg/L)+ZT(0.5 mg/L).The inducement rate of valid buds reached to 74.5%.The regenerated shoot from leaf was obtained when cultured on 1/2MS+TDZ(1.0 mg/L)+IAA(0.1 mg/L)+6-BA(2.0 mg/L) for 7 weeks.(2) Regenerated shoot were cut as several cluster or single plantlet and transferred on 1/2MS+6 BA(1.0 mg/L)+NAA(0.05 mg/L) for shoot propagation and growth,which the multiplication coefficient was more than 4.17.(3) 88.3% of rooting rate,3.72 cm of average root length,and 8 roots of average plantlet were observed when cultured in 1/2 MS for 50 days,after shoot was dipped in 1 g/L IBA for 5 second.(4) The rooted plantlets were acclimatized on mixture of peat and vermiculite(1:1),which survival rate was 82.5%.