Analysis of Irregular Antibodies Detected in Different Types of Voluntary Blood Donors

Objective To explore irregular antibody detection rate and distribution of different types of blood donors,in order to improve the clinical transfusion safety.Methods From January 2004 to December 2012,a total of 175 587 cases of blood donors in Blood Center of Dongying were included in this study.Male donors were 121 682 cases,and female were 53 905 cases.Donors' ages were 18 to 55 years old,and average ages were (33.246.5) years old.All donors were conformed by the Requirements of Donor Health Examination (GB18467-2001 or GB18467-2011).Informed consent was obtained from all donors before blood collection.Saline agglutination method was used to screen irregular antibodies of blood donors.Indirect anti globulin experiment and spectrum cell screening method were used to confirm positive results.Results Among these 175 587 cases of blood donors,103 cases were detected out irregular antibodies,with the rate of 0.059 ％.The irregular antibody detection rate in different bloods donors were 0.076％ in O blood type donors,0.051％ in A blood type,0.052％ in B blood type,and 0.060％ in AB blood type,respectively.Irregular antibody detection rate had no statistically significant difference among different blood types donors (x2 =3.34,P＞0.05).The irregular antibody detection rate in different ages donors were 0.029％ in 18-25 years old,0.062％ in ＞25-35 years old,0.103％ in ＞35-45 years old and 0.134％ in ＞ 45-55 years old,respectively.The difference of irregular antibody detection rate had statistically significant among different ages donors (x2 =31.38,P＜0.01).Women's irregular antibody detection rate was 0.082％,which was higher than 0.048％ of men (x2=7.00,P＜0.01).There were 9 cases in Rh blood group system (50.0％,9/18) and 7 cases in MNS blood group system in 18 donors who were detected out irregular antibody.Conclusions With the increasing of blood donors' age,the irregular antibody detection rate increases.Anti-E and anti-M irregular antibodies are more common.We should pay more attention to irregular antibody screening of over 35 years old female recipients,in order to improve the safety of clinical blood transfusion. Key words: Blood Donors;  Antibody;  Blood group system