The significance of irregular antibody screening among healthy blood donors for clinical blood transfusion

Objective To investigate the significance of screening irregular antibodies among healthy blood donors for clinical blood transfusion.Methods 3 029 blood samples of healthy donors from Augst 2011 to October 2012 were collected.Saline medium method and Coombs test were used for irregular antibodies screening.The positive samples of the screening tests were further evaluated to analyze their antibody specificity.Results Among the 3 029 healthy donors,17 cases(0.56%) were positive for irregular antibodies screening,including 9 cases for anti-D antibody,4 cases for anti-E antibody,1 case for anti-e antibody,2 cases for anti-M antibody and 1 case for anti-A1 antibody respectively.The positive rates of male and female were 0.43%(6/1 397) and 0.67%(11/1 632).The difference had no statistical significance(P0.05).The positive rate of irregular antibody in healthy donors who had history of blood transfusion or pregnancy was significantly higher than that in healthy donors without history of blood transfusion or pregnancy(1.48 % vs 0.1%,P0.05).Conclusion It might be with great significance to conduct routine screening and identification of irregular antibody among healthy donors,which could ensures clinical transfusion safety and prevent hemolytic transfusion reaction.