2011•Chin J Naut Med & Hyperbar MedRequires access
Effect of hyperbaric oxygen on the anti-senility of mice with Alzheimer disease
Zhou Hong-tu, Lirong Yan, Jian-guo Yuan, Rong Li, Xuechun Zhang, Yumei Zhou
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Abstract
Objective To study the effect of hyperbaric oxygen on the anti-senility of mice with Alzheimer disease.Methods Thirty male KM mice were randomly divided into 3 groups:the control group,the model group and the HBO group.Models of dementia mice were successfully developed with D-galactose (120 mg/kg) and sodium nitrite (90 mg/kg).Escape latency and times of running across the platform were respectively determined by Morris water maze.The activity of serum superoxide dismutase (SOD),glutathione peroxide (GSH-Px) and the content of malondialdehyde (MDA) were detected with biochemical enzyme and thiobarbituric acid.Morphology of neurons in the hippocampal CA1 region was observed with H&E staining.Results When compared with that of the control group,the escape latency for the model group obviously prolonged [ (76.13 ± 25.28 ) s ] (P ＜ 0.01 ),times of running across the platform reduced [ ( 2.34 ± 0.87 )times ] (P ＜ 0.01 ),the activity of SOD [ ( 269.70 ± 33.45 ) U/ml ] and GSH-Px [ (4.38 ± 1.21 ) g/L ]significantly decreased,but the content of MDA increased [ (6.57 ±2.04) μmol/L] (P ＜0.01 ).A great amount of degenerative neurons and sparse irregular cell arrangements could be detected in hippocampal CA1region in the dementia mice.Following HBO treatment,the escape latency shortened,the times of running across the platform increased,the activity of SOD and GSH-Px significantly increased,while the content of MDA decreased,with statistical significance (P ＜0.01 ).The No.of normal cells in the hippocampal CA1 region increased significantly,when compared with that of HBO treatment,and the neurons in the CA1 region were tightly arranged.Conclusions HBO could obviously increase the capacity of the mice with AD to alleviate lesion induced by oxygen free radicals and has certain effect on the treatment of AD through anti-senile mechanism.
Key words:
Hyperbaric oxygen; Alzheimer disease; Superoxide dismutase (SOD); Malondialdehyde (MDA) ; Glutathione; Oxidative stress