Study of effect of Oncolyn on oxidative damage caused by galactose in senescent mice

Objective To investigate the neutralization effect of Oncolyn on oxidative damage in aging mice.To explore the effect of Oncolyn on the oxidative damage caused by D-galactose in aging mice.Method The Kun-ming mice were divided into three groups:control group,aging mice group and Oncolyn intervening group.The senescence model was made by injecting of D-galactose for 22 days,Oncolyn were administered by oral for 70 days from the 22nd day after injection of galactose.The levels of SOD,GSH-Px,NO,NOS,MDA,MAO in blood and brain tissue were detected,the activities of ATPase in myocardial tissues were also measured.Additionally,the expression of Caspase-3 in pallium and hippocampus was measured too,using immunohistochemical technique.Result Compared with the control group,the activities of SOD and GSH-Px in blood and brain tissue were all lowered in senescent mice,the levels of NO and NOS were higher in blood and lowered in brain tissue(P0.05),and the levels of MDA and MAO,the expression of Caspase-3 in senescent mice rose than that in control group.In Oncolyn group,the activities of SOD and GSH-Px in blood were 190.31 U/ml and 163.15 U/ml respectively,were higher than that of aging group(were 143.19 U/ml and 105.81 U respectively,P0.05),the change trends of SOD and GSH-Px in brain tissue were the same like that in blood,but the activity of NOS was higher in brain tissue,not lowered like that in blood.Additionally,the levels of MDA and MAO and the expression of Caspase-3 also were lower both in pallium and hippocampus of the mice from Oncolyn group compared with aging group.Conclusion Oncolyn could enhance the ability of anti-oxidation,thereby could remit the process of aging.