2014•Traditional Chinese MedicineRequires access

Determination of hyperoside in Bushen-Yijing wan by Ion-Pair HPLC

王正军, 吕明

Abstract

Objective To establish a HPLC method for the determination of Hyperoside in Bushen-Yijing wan. Methods The HPLC system consisted of the Agilent HC-C18（4.6 mm×250 mm, 5μm）column was adopted. The mobile phase was consisted of acetonitrile（10）-HK2PO4（90）（0.85 g/L sodium heptanesulfonate,H3PO4 adjusted pH to 4.8）, the flow rate was 1.0 ml/min, the column temperature was 30 ℃, and the UV detector was set at 360 nm. Results The linear response range was 3.0~60.0μg/ml（r=0.999 9）. The average recovery of hyperoside was 98.2%, and RSD1.53%. Conclusions The method is simple, rapid, accurate and repeatable. It can be applied in determination of Hyperoside in Bushen-Yijing wan. Key words: Hyperoside; Bushen-Yijing wan; Ion-Pair HPLC

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Key concepts: Hyperoside, Chromatography, Chemistry, High-performance liquid chromatography, Acetonitrile, Sodium, Biochemistry, Quercetin