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2014•Guiding Journal of Traditional Chinese Medicine and PharmacyRequires access

Determination of Hyperoside in Jiawei Shuilu Wan by HPLC

Yang Hai-l

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Abstract

Objective:To establish a HPLC method for the determination of Hyperoside in Jiawei Shuilu wan.Methods:The HPLC system consisted of the DIONEX-C18(4.6 mm×250 mm,5 μm)column,the mobile phase consisted of acetonitrile(10)-0.1% H3PO4(90);the flow rate was 1.0 mL·min-1;the column temperature was 30 ℃;The UV detector was set at 360 nm.Results:The linear response range was 4.0-80.0 μg/mL(r=0.9998).The average recovery of hyperoside was 97.6%,and RSD% =1.26%.Conclusions:The method is simple,rapid,accurate and repeatable.It can be applied in determination of Hyperoside in Jiawei Shuilu wan.

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What this paper is about

Objective:To establish a HPLC method for the determination of Hyperoside in Jiawei Shuilu wan.Methods:The HPLC system consisted of the DIONEX-C18(4.6 mm×250 mm,5 μm)column,the mobile phase consisted of acetonitrile(10)-0.1% H3PO4(90);the flow rate was 1.0 mL·min-1;the column temperature was 30 ℃;The UV detector was set at 360 nm.Results:The linear response range was 4.0-80.0 μg/mL(r=0.9998).The average recovery of hyperoside was 97.6%,and RSD% =1.26%.Conclusions:The method is simple,rapid,accurate and repeatable.It can be applied in determination of Hyperoside in Jiawei Shuilu wan.

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Available abstract

Objective:To establish a HPLC method for the determination of Hyperoside in Jiawei Shuilu wan.Methods:The HPLC system consisted of the DIONEX-C18(4.6 mm×250 mm,5 μm)column,the mobile phase consisted of acetonitrile(10)-0.1% H3PO4(90);the flow rate was 1.0 mL·min-1;the column temperature was 30 ℃;The UV detector was set at 360 nm.Results:The linear response range was 4.0-80.0 μg/mL(r=0.9998).The average recovery of hyperoside was 97.6%,and RSD% =1.26%.Conclusions:The method is simple,rapid,accurate and repeatable.It can be applied in determination of Hyperoside in Jiawei Shuilu wan.

Key concepts: Hyperoside, Chromatography, High-performance liquid chromatography, Chemistry, Acetonitrile, Linear range, Detection limit, Quercetin

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