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2015•Journal of Hubei University of Chinese MedicineRequires access

Determination of Hyperoside in Fu'an Granule by HPLC

LI Zhi-ha

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Abstract

Objective To establish a HPLC method for the determination of hyperoside in Fu'an granule. Method The HPLC system consisted of the Fortis C18( 4. 6mm × 250 mm,5μm) column,the mobile phase consisted of acetonitrile- 0. 1% H3PO4( 14: 86),the flow rate was 1. 0m L / min,the column temperature was 30℃,the UV detector was set at 360 nm. Results The linear response range was 1. 8- 36. 0μg /m L( r = 0. 9999). The average recovery of hyperoside was 99. 2%,and RSD was 1. 18%. Conclusions The method is simple,rapid,accurate and repeatable. It can be applied in determination of hyperoside in Fu'an granule.

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What this paper is about

Objective To establish a HPLC method for the determination of hyperoside in Fu'an granule. Method The HPLC system consisted of the Fortis C18( 4. 6mm × 250 mm,5μm) column,the mobile phase consisted of acetonitrile- 0. 1% H3PO4( 14: 86),the flow rate was 1. 0m L / min,the column temperature was 30℃,the UV detector was set at 360 nm. Results The linear response range was 1. 8- 36. 0μg /m L( r = 0. 9999). The average recovery of hyperoside was 99. 2%,and RSD was 1. 18%. Conclusions The method is simple,rapid,accurate and repeatable. It can be applied in determination of hyperoside in Fu'an granule.

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Available abstract

Objective To establish a HPLC method for the determination of hyperoside in Fu'an granule. Method The HPLC system consisted of the Fortis C18( 4. 6mm × 250 mm,5μm) column,the mobile phase consisted of acetonitrile- 0. 1% H3PO4( 14: 86),the flow rate was 1. 0m L / min,the column temperature was 30℃,the UV detector was set at 360 nm. Results The linear response range was 1. 8- 36. 0μg /m L( r = 0. 9999). The average recovery of hyperoside was 99. 2%,and RSD was 1. 18%. Conclusions The method is simple,rapid,accurate and repeatable. It can be applied in determination of hyperoside in Fu'an granule.

Key concepts: Hyperoside, Chromatography, Granule (geology), High-performance liquid chromatography, Chemistry, Acetonitrile, Linear range, Materials science

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