2004•Chin J EndemiolRequires access

Protective immunity against Schistosoma japonicum induced in mice immunized with recombinant 32×103 protein

W.-H. Yao L. Min, 曾宪芳, 章洁, 李先平, 张顺科, 易新元

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Abstract

目的 用重组日本血吸虫32×103蛋白(rSj32×103)免疫Balb/c鼠,检测特异性IgG抗体水平,并观察抗日本血吸虫的保护效果.方法用rSj32×103加佐剂免疫小鼠3次,分别在0、2、4周进行,第6周每鼠经腹部感染(40±1)条日本血吸虫尾蚴,感染后42d剖杀,计算减虫率.并用ELISA方法检测免疫后的抗体反应.结果间接ELISA测得免疫鼠特异性IgG抗体滴度达1:12800.与对照组相比,减虫率为21.9%.结论用rSj32×103免疫小鼠后可诱导特异性IgG抗体,并能发挥一定的免疫保护作用。

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目的 用重组日本血吸虫32×103蛋白(rSj32×103)免疫Balb/c鼠,检测特异性IgG抗体水平,并观察抗日本血吸虫的保护效果.方法用rSj32×103加佐剂免疫小鼠3次,分别在0、2、4周进行,第6周每鼠经腹部感染(40±1)条日本血吸虫尾蚴,感染后42d剖杀,计算减虫率.并用ELISA方法检测免疫后的抗体反应.结果间接ELISA测得免疫鼠特异性IgG抗体滴度达1:12800.与对照组相比,减虫率为21.9%.结论用rSj32×103免疫小鼠后可诱导特异性IgG抗体,并能发挥一定的免疫保护作用。

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Available abstract

目的 用重组日本血吸虫32×103蛋白(rSj32×103)免疫Balb/c鼠,检测特异性IgG抗体水平,并观察抗日本血吸虫的保护效果.方法用rSj32×103加佐剂免疫小鼠3次,分别在0、2、4周进行,第6周每鼠经腹部感染(40±1)条日本血吸虫尾蚴,感染后42d剖杀,计算减虫率.并用ELISA方法检测免疫后的抗体反应.结果间接ELISA测得免疫鼠特异性IgG抗体滴度达1:12800.与对照组相比,减虫率为21.9%.结论用rSj32×103免疫小鼠后可诱导特异性IgG抗体,并能发挥一定的免疫保护作用。

Key concepts: Schistosoma japonicum, Immunity, Recombinant DNA, Virology, Biology, Humoral immunity, Immunology, Schistosomiasis

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