Study on protective cell immune mechanism of recombinant 31/32kDa antigens of Schistosoma japanicum

Objective To study the effect of cell immunity in the protective immune mechanism of recombinant rSj31/32kDa antigens of Schistosoma japonicum(rSj31/32).Methods BALB/c mice were immunized three times with rSj31/32.ELISA kits were used to examine the levels of IFN-γ and IL-4 before and 6 weeks after immunization.By the culture of spleen cells after challenge,IFN-γ and IL-4 after stimulation with SEA were quantified by ELISA kits.Results The level of IFN-γ were increased significantly after immunized with rSj31/32(P0.001).There was no obvious change in the level of IL-4.With spleen cells from rSj31/32 vaccinated mice,IFN-γ was the most abundantly produced cytokine in response to SEA,while IL-4 secretion was the lowest(P0.01).Conclusion Cell immunity might play an important role in the protective immunity elicited by rSj31/32 antigen.