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Combined application of PCR-SSP and PCR-SSCP in bone marrow transplantation typing

孙玉英, 孔繁华, 奚永志, 刘广贤, 陈兴国, 金荔, 屠敏

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Abstract

Objective To study the method for human leukocyte antigen (HLA) typing. Methods HLA genotyping was performed by using PCR-SSP and PCR-SSCP in 20 pairs of bone marrow transplantation donor-recipients who underwent serologic typing. DNA from 20 pairs of samples were extracted by a fast salting out method. Results The serological and genotyping results were identical in 18 cases, serological typing was failure in 2 cases who could be typed easily by genotyping methods. Between the two genotyping methods, there were 19 cases of identical results and 1 case of different results. After the patient received the graft, he got IV grade graft versus host disease (GVHD). Conclusion The combined application of PCR-SSP and PCR-SSCP was an ideal typing method and might be helpful to accurately tpye HLA. Key words: HLA antigens; Immunoassay; Polymerase chain reaction

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What this paper is about

Objective To study the method for human leukocyte antigen (HLA) typing. Methods HLA genotyping was performed by using PCR-SSP and PCR-SSCP in 20 pairs of bone marrow transplantation donor-recipients who underwent serologic typing. DNA from 20 pairs of samples were extracted by a fast salting out method. Results The serological and genotyping results were identical in 18 cases, serological typing was failure in 2 cases who could be typed easily by genotyping methods. Between the two genotyping methods, there were 19 cases of identical results and 1 case of different results. After the patient received the graft, he got IV grade graft versus host disease (GVHD). Conclusion The combined application of PCR-SSP and PCR-SSCP was an ideal typing method and might be helpful to accurately tpye HLA. Key words: HLA antigens; Immunoassay; Polymerase chain reaction

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Available abstract

Objective To study the method for human leukocyte antigen (HLA) typing. Methods HLA genotyping was performed by using PCR-SSP and PCR-SSCP in 20 pairs of bone marrow transplantation donor-recipients who underwent serologic typing. DNA from 20 pairs of samples were extracted by a fast salting out method. Results The serological and genotyping results were identical in 18 cases, serological typing was failure in 2 cases who could be typed easily by genotyping methods. Between the two genotyping methods, there were 19 cases of identical results and 1 case of different results. After the patient received the graft, he got IV grade graft versus host disease (GVHD). Conclusion The combined application of PCR-SSP and PCR-SSCP was an ideal typing method and might be helpful to accurately tpye HLA. Key words: HLA antigens; Immunoassay; Polymerase chain reaction

Key concepts: Genotyping, Typing, Serology, Polymerase chain reaction, Human leukocyte antigen, Tissue typing, Transplantation, Histocompatibility Testing

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