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1999•Chineae Journal of Organ TransplantationRequires access

Combined application of PCRSSP and PCRSSCP in bone marrow transplantation typing

Yongzhi Xi

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Abstract

Objective To study the method for human leukocyte antigen (HLA) typing. Methods HLA genotyping was performed by using PCRSSP and PCRSSCP in 20 pairs of bone marrow transplantation donorrecipients who underwent serologic typing. DNA from 20 pairs of samples were extracted by a fast salting out method. Results The serological and genotyping results were identical in 18 cases, serological typing was failure in 2 cases who could be typed easily by genotyping methods. Between the two genotyping methods, there were 19 cases of identical results and 1 case of different results. After the patient received the graft, he got grade graft versus host disease (GVHD). Conclusion The combined application of PCRSSP and PCRSSCP was an ideal typing method and might be helpful to accurately tpye HLA.

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What this paper is about

Objective To study the method for human leukocyte antigen (HLA) typing. Methods HLA genotyping was performed by using PCRSSP and PCRSSCP in 20 pairs of bone marrow transplantation donorrecipients who underwent serologic typing. DNA from 20 pairs of samples were extracted by a fast salting out method. Results The serological and genotyping results were identical in 18 cases, serological typing was failure in 2 cases who could be typed easily by genotyping methods. Between the two genotyping methods, there were 19 cases of identical results and 1 case of different results. After the patient received the graft, he got grade graft versus host disease (GVHD). Conclusion The combined application of PCRSSP and PCRSSCP was an ideal typing method and might be helpful to accurately tpye HLA.

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Available abstract

Objective To study the method for human leukocyte antigen (HLA) typing. Methods HLA genotyping was performed by using PCRSSP and PCRSSCP in 20 pairs of bone marrow transplantation donorrecipients who underwent serologic typing. DNA from 20 pairs of samples were extracted by a fast salting out method. Results The serological and genotyping results were identical in 18 cases, serological typing was failure in 2 cases who could be typed easily by genotyping methods. Between the two genotyping methods, there were 19 cases of identical results and 1 case of different results. After the patient received the graft, he got grade graft versus host disease (GVHD). Conclusion The combined application of PCRSSP and PCRSSCP was an ideal typing method and might be helpful to accurately tpye HLA.

Key concepts: Genotyping, Typing, Serology, Tissue typing, Human leukocyte antigen, Bone marrow transplantation, Histocompatibility Testing, Medicine

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