HLA-AB gene assignment by PCR-SSP technique and comparison of serological and DNA typing results

Objective To develop the method for DNA typing of HLA class Igenome Methods HLA A and HLA B typing were performed by PCR SSP technique and a comparison of the typing results was carried out between serology and PCR SSP. Results Two recipients were compatible to HLA matching donors in 22 patients and 48 donors,and one of them succeeded in bone marrow transplantation(BMT).Of the 70 samples,43(61.4%)of the PCR SSP typings were in agreement with serology.However,there were 27 (38.6%)of 70 samples discrepancies between the serology typings and the molecular results.Conclusion The typing method of PCR SSP is accurate,simple and rapid,it might replace the serological testing for HLA