1999•Zhongguo mianyixue zazhiRequires access

Method of HLA B27 genotyping

Lan Jiong

Abstract

Objective:A comparison study of polymerase chain reaction (PCR) and serological typing method was used to detect HLA B27.Methods:78 samples from patients suspected with ankylosing spondylitis (AS) were genotyped by PCR and some of them were assayed by serological typing.Results:24/36 samples with HLA B27 gene were compared with serological typing ,the results of 5 samples serological typing were dubious or contradictory with PCR genotyping.21/42 samples without HLA B27 gene were compared with serological typing,the results of 3 samples serological typing were dubious.Conclusion:The method of B27 genotyping by PCR is more rapid,inexpensive and exact than phenotyping by serology.

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Key concepts: Genotyping, Typing, Serology, Polymerase chain reaction, HLA-B27, Biology, Human leukocyte antigen, Genotype